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Sharing this short video from @SpringerNature, which showcases our work on ApoB, the protein at the heart of LDL cholesterol and heart disease, alongside the Nobel Prize-winning work from the AlphaFold team at @GoogleDeepMind! Big shout out to my @Mizzou collaborator @zacharyberndsen!
What if the key to heart disease lies in a single protein?
Our video follows ApoB, once impossible to map, now revealed in minutes with AI like AlphaFold. A breakthrough showing how AI is accelerating science & unlocking treatments.
Watch the video: spklr.io/6019EMI0P
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C. Keith Cassidy retweeted
April's Molecule of the Month: Apolipoprotein B-100 and LDL Receptor
Insights into how LDL is removed from the bloodstream
pdb101.rcsb.org/motm/304
Insights into how LDL is removed from the bloodstream
pdb101.rcsb.org/motm/304
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Our ApoB paper is out in print today in @Nature and since we didn't get the cover I can share our (@CKeithCassidy ) beautiful artwork from molgraphics.com that wasn't selected 😆
rdcu.be/eaGPH
rdcu.be/eaGPH
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Insights into how cholesterol-carrying low-density lipoproteins are structured, and how they interact with receptors to enter cells, could help to clarify the role of these particles in cardiovascular disease
nature.com/articles/d4158…
nature.com/articles/d4158…
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C. Keith Cassidy retweeted
The first independent publication from the Berndsen lab @Mizzou_Biochem in collaboration with my colleague @CKeithCassidy is now peer reviewed and online at @Nature !! rdcu.be/d3kfg
Resolving a many decades-old challenge, we determined the complete structure of the surface of its native #LDL particle using a combination single-particle #cryoEM, #AlphaFold2, and molecular dynamics refinement methods. At ~550 kDa, with ~4.6k residues of mostly extended amphipathic domains, #ApoB is one of the largest and most complex proteins in the genome. Our structure provides insight into lipoprotein synthesis, the structural changes involved in the catabolic conversion from VLDL to LDL, and the nature of disease causing mutations involved in diseases such as #FamilialHypercholesterolemia.
The main change from the preprint is the addition of extensive chemical crosslinking data, which validate many of the lower-resolution contacts we observed between the C-terminal inserts and lends support to our proposed model for conformational changes on larger LP particles.
With the structure of apoB finally revealed, a new era in lipoprotein research begins were highly precise and targeted structural, genetic, and computational studies are now possible! We have many plans and are excited for what the future of this field holds, so stayed tuned!!
Resolving a many decades-old challenge, we determined the complete structure of the surface of its native #LDL particle using a combination single-particle #cryoEM, #AlphaFold2, and molecular dynamics refinement methods. At ~550 kDa, with ~4.6k residues of mostly extended amphipathic domains, #ApoB is one of the largest and most complex proteins in the genome. Our structure provides insight into lipoprotein synthesis, the structural changes involved in the catabolic conversion from VLDL to LDL, and the nature of disease causing mutations involved in diseases such as #FamilialHypercholesterolemia.
The main change from the preprint is the addition of extensive chemical crosslinking data, which validate many of the lower-resolution contacts we observed between the C-terminal inserts and lends support to our proposed model for conformational changes on larger LP particles.
With the structure of apoB finally revealed, a new era in lipoprotein research begins were highly precise and targeted structural, genetic, and computational studies are now possible! We have many plans and are excited for what the future of this field holds, so stayed tuned!!
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BREAKING NEWS
The Royal Swedish Academy of Sciences has decided to award the 2024 #NobelPrize in Physics to John J. Hopfield and Geoffrey E. Hinton “for foundational discoveries and inventions that enable machine learning with artificial neural networks.”
The Royal Swedish Academy of Sciences has decided to award the 2024 #NobelPrize in Physics to John J. Hopfield and Geoffrey E. Hinton “for foundational discoveries and inventions that enable machine learning with artificial neural networks.”
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C. Keith Cassidy retweeted
BREAKING NEWS
The Royal Swedish Academy of Sciences has decided to award the 2024 #NobelPrize in Chemistry with one half to David Baker “for computational protein design” and the other half jointly to Demis Hassabis and John M. Jumper “for protein structure prediction.”
The Royal Swedish Academy of Sciences has decided to award the 2024 #NobelPrize in Chemistry with one half to David Baker “for computational protein design” and the other half jointly to Demis Hassabis and John M. Jumper “for protein structure prediction.”
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In our article published today @Nature , we report complete all-atom structural models of a fully packaged bacteriophage particle. Congratulations to Kush Coshic on his first publication! Many thanks to Chris Maffeo and David Winogradoff for pushing this 6-year project forward.
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New preprint with @zacharyberndsen on the structure of apoB100 from human LDL! Special thanks to @Mizzou Physics and IT/RSS that were instrumental in getting my new lab up and running!
I am thrilled to share the first preprint from my lab at @Mizzou_Biochem! My colleague @CKeithCassidy and I determined the structure of apoB100 from human low-density lipoprotein (LDL). This protein is wild!
biorxiv.org/content/10.110…
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My name is Alise Muok and I just published my first fiction book! Six Fantasy/SciFi/Horror tales with illustrations and music included. Curious? You can take a look here:
barnesandnoble.com/w/books/114400…
barnesandnoble.com/w/books/114400…
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CheA histidine kinase, the "brain" of bacterial chemosensory systems, is a five-domain protein, as seen in E. coli and B. subtilis.
Well, more often than not, this is not true!
biorxiv.org/content/10.110…
Well, more often than not, this is not true!
biorxiv.org/content/10.110…
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Advertising an MRC-funded DTP PhD in my brand new lab at Bristol University, using molecular modelling (and some expts) to understand the role of the membrane on µ-opioid receptor activity. RTs welcome. gw4biomed.ac.uk/how-does-the-m…
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Having this work published is a symbol of perseverance. From getting married, taking a leave for health, and moving to a different country, this work was there the whole way through! Thank you @BriegelAriane @CKeithCassidy @flory_olsthoorn @ortega_science journals.asm.org/doi/10.1128/mb…
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Making more progress on the T4 phage illustration. All the mRNA (viral and cellular) is in, added most of the cell wall, and just finished adding a bunch of chaperonins. Now I need to add lots and lots and lots of soluble molecules.
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Keren (@LaskerKeren) and I describe the science behind our recent Caulobacter illustration in "Integrative Visualization of the Molecular Structure of a Cellular Microdomain", now online ahead of print at Protein Science: onlinelibrary.wiley.com/doi/full/10.10…
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Excited to share our most recent work on the giant ubiquitin ligase #BIRC6 out now in @ScienceMagazine science.org/doi/10.1126/sc…!
Thread below 🧵 (1/7)
Thread below 🧵 (1/7)
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Dr. Keith Cassidy @CKeithCassidy is taking the stage! Exciting science talk on ‘Resolving new structural features of the E. coli chemotaxis core signaling unit’ combining molecular dynamics simulation, cryo-ET, AlphaFold2. #blastmeeting2023 @Blastmeeting
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