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This preprint is now an official, peer-reviewed paper 🥳
Microscopic phage adsorption assay: High-throughput quantification of virus particle attachment to host bacterial cells
pnas.org/doi/10.1073/pn…
Tweetorial at the other place: bsky.app/profile/did:pl…
Microscopic phage adsorption assay: High-throughput quantification of virus particle attachment to host bacterial cells
pnas.org/doi/10.1073/pn…
Tweetorial at the other place: bsky.app/profile/did:pl…
Finally, the preprint on my postdoc work from the past 3 years! With mentorship from @PaulTurnerLab and @EmonetLab, I developed a microscopic technique to measure attachment of viruses #phages to bacterial cells @YalePhage @QbioYale .
biorxiv.org/content/10.110…
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Jyot Antani retweeted
Evolutionary consequences of bacterial resistance to a flagellotropic phage biorxiv.org/content/10.110… #biorxiv_evobio
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Jyot Antani retweeted
New tool offers insights into #virus-cell interactions ...
| #antibacterial | #Bacteriophages | #phage | #virus | Via Yale .com
buff.ly/IcoUfim
| #antibacterial | #Bacteriophages | #phage | #virus | Via Yale .com
buff.ly/IcoUfim
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Jyot Antani retweeted
.@AntaniJo (@Yale) etal developed a microscopy-based technique to measure #phage attachment to bacterial cells. This method provides an alternative to the century-old Phage Adsorption Assay. Feel free to reach out with any questions: jyot.antani@yale.edu.
buff.ly/4fd1PnD
buff.ly/4fd1PnD
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Jyot Antani retweeted
💀 According to our survey, over 60% of @Yale #postdocs are receiving compensation below the 2024 minimum!
The second one was a trick question!
💀A staggering 85% of @Yale #postdocs are dissatisfied with their compensation.
@yaledailynews
The second one was a trick question!
💀A staggering 85% of @Yale #postdocs are dissatisfied with their compensation.
@yaledailynews
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Repeat after me: Virus traits are important drivers of community dynamics.
Cool preprint showing that a cocktail of two phages with similar adsorption rates but different latent periods essentially has the same effect as a single phage (one with the shorter latent period).
Cool preprint showing that a cocktail of two phages with similar adsorption rates but different latent periods essentially has the same effect as a single phage (one with the shorter latent period).
🎉Our preprint illustrates the integration of experimental and computational approaches to study phage combinations for cocktail optimization. @KiranKondabagil
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Jyot Antani retweeted
Having lots of Pf phage, a phage that partners with Pseudomonas to build biofilms, in your sputum leads to a decline in lung function over time in people with cystic fibrosis. Congrats to @ebburgenerMD, @PRSecor and Carlos Milla on this lovely work. sciencedirect.com/science/articl…
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Jyot Antani retweeted
“I propose the R-Index, defined as the difference between the sum of review responsibilities for a researcher’s publications and the number of reviews they have completed, as a novel metric…”
arxiv.org/abs/2407.19949
arxiv.org/abs/2407.19949
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Why use a SHOTGUN when the SITUation can be RESEOLVED with a SPATIAL op?
The advances in sequencing technologies are absolutely amazing! 🤩
The advances in sequencing technologies are absolutely amazing! 🤩
Unveiling Expansion in situ genome sequencing: a revolutionary leap in spatial genomics! ExIGS enables simultaneous nanoscale mapping of gDNA and proteins in single cells.
It's been an incredible journey co-leading this work with @z_chiang , @CarolineComenho , @JD_Buenrostro
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Jyot Antani retweeted
Another step forward towards the post-plaque era! Great work helping to drag the phage field into the 21st Century. Many core methodologies we use to study phages haven't changed since they were invented almost 100 years ago
Finally, the preprint on my postdoc work from the past 3 years! With mentorship from @PaulTurnerLab and @EmonetLab, I developed a microscopic technique to measure attachment of viruses #phages to bacterial cells @YalePhage @QbioYale .
biorxiv.org/content/10.110…
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This method provides an alternative to the (literally) century-old Phage Adsorption Assay because it...
- is fast
- consumes less media
- provides single-virus readouts
- does not rely on a theoretical model to estimate adsorption rate
- is fast
- consumes less media
- provides single-virus readouts
- does not rely on a theoretical model to estimate adsorption rate
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Hope this will help the fields of phage biology, microbial technology, and phage therapy.
@phagepapers @phagedirectory @VoM_2024
@phagepapers @phagedirectory @VoM_2024
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Finally, the preprint on my postdoc work from the past 3 years! With mentorship from @PaulTurnerLab and @EmonetLab, I developed a microscopic technique to measure attachment of viruses #phages to bacterial cells @YalePhage @QbioYale .
biorxiv.org/content/10.110…
biorxiv.org/content/10.110…
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Jyot Antani retweeted
Microscopic Phage Adsorption Assay: High-throughput quantification of virus particle attachment to host bacterial cells biorxiv.org/cgi/content/sh… #biorxiv_micrbio
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As an international postdoc, you can't get grants which are a sign of competence for faculty jobs.
And good luck if you were born in India. You will never get a green card so no industry career for you.
You will always be a postdoc.
And good luck if you were born in India. You will never get a green card so no industry career for you.
You will always be a postdoc.
International postdocs in the U.S. are short-changed—in more ways than one | Science - The study is “super valuable,” says Andrea Pereyra, assistant director of postdoctoral life design at Johns Hopkins University who moved to the ... - ift.tt/05tpGFi
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Jyot Antani retweeted
Preprint alert 🚨! I present you the second and final manuscript on the BASEL phages! 36 new deeply characterized phages from viral groups that were so far mostly terra incognita - and all infect the E. coli K12 lab strain with easy tricks. 1/12
tinyurl.com/3xdt65f5
tinyurl.com/3xdt65f5
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Jyot Antani retweeted
It took more than a DECADE... but finally it’s published!!! It started as a CRAZY idea but after tons of hard work we built it: We hijacked the endogenous secretion systems of Toxoplasma gondii, a brain parasite, to deliver active proteins to neurons 👇
nature.com/articles/s4156…
nature.com/articles/s4156…
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Jyot Antani retweeted
Please come check out my talk tomorrow, where I’ll talk about some of the early results from my evolution experiment. See you there!
#Evol2024
#Evol2024
@NoahHoupt (Sat, 2:45-3PM, 524B) is discussing his 1,000-generation evolution experiment exploring how niche construction impacts long-term evolutionary dynamics. Prelim results demonstrate the rapid evolution of adaptive niche construction driven by a surprising mechanism 😱.
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Jyot Antani retweeted
Super excited to share my postdoc work on Monday at #Evol2024! Come hear about the role of a prophage in the thermal eco evo of a strain I collected in the Long Island Sound 🔥🧫🌊
Postdoc @_cathyhernandez (Mon, 10:15-10:30AM, 516E) will present her work showing how the thermal ecology and evolution of a marine bacterium is shaped by the temperature-dependence of an integrated phage 🌊.
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Jyot Antani retweeted
Hey #Evol2024! Interested in the evolutionary ecology of host-parasite interactions? Come check out the presentations by my amazing trainees, listed below!
I'll give a brief preview of each presentation below 🧵
I'll give a brief preview of each presentation below 🧵
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