Parallel Squared Technology Institute (PTI) retweeted
In pursuit of this new direction, our team at @ParallelSqTech developed multiple timePlex implementations.
They demonstrate strong potential. A few highlights include:
biorxiv.org/content/10.110…
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They demonstrate strong potential. A few highlights include:
biorxiv.org/content/10.110…
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Parallel Squared Technology Institute (PTI) retweeted
Participating in the rapid evolution of this technology, from proof of principle to a robust, quantitative methodology, has been a thrilling adventure.
Writing this perspective revived many wonderful memories and brought a bright smile to my face.
Writing this perspective revived many wonderful memories and brought a bright smile to my face.
New ACS Editors' Choice from @J_ASMS!
Congratulations to Nikolai Slavov! His Perspective explores a decade of advances in single-cell proteomics and the new possibilities they're opening for biology.
Read more: doi.org/10.1021/jasms.…
#JASMS #TeamMassSpec #MassSpectrometry
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Parallel Squared Technology Institute (PTI) retweeted
Realizing this workflow for single-cell proteomics benefited from discussions at the 2025 single-cell proteomics conference.
Facilitating such collaborations is a core part of @SCP_meeting's mission !
Facilitating such collaborations is a core part of @SCP_meeting's mission !
Parallel Squared Technology Institute aims to scale the throughput of sensitive proteomics without compromising quantitative accuracy, depth or specificity.
Today, we share a step in this direction:
🔹 A workflow supporting the analysis of 1,130 single-cell samples per day depth and quantitative accuracy.
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biorxiv.org/content/10.110…
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Parallel Squared Technology Institute (PTI) retweeted
𝐇𝐨𝐰 𝐝𝐢𝐝 𝐰𝐞 𝐝𝐨 𝐢𝐭 ?
There are two broad routes to higher throughput mcponline.org/retrieve/pii/S…: Each run can be shortened, which squeezes the same peptides into less time and generally strains peptide separation and thus depth and quantification. Alternatively, one can measure several samples in each run. We took the second route, which can be combined with fast chromatography.
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There are two broad routes to higher throughput mcponline.org/retrieve/pii/S…: Each run can be shortened, which squeezes the same peptides into less time and generally strains peptide separation and thus depth and quantification. Alternatively, one can measure several samples in each run. We took the second route, which can be combined with fast chromatography.
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Parallel Squared Technology Institute (PTI) retweeted
Parallel Squared Technology Institute aims to scale the throughput of sensitive proteomics without compromising quantitative accuracy, depth or specificity.
Today, we share a step in this direction:
🔹 A workflow supporting the analysis of 1,130 single-cell samples per day depth and quantitative accuracy.
1/
biorxiv.org/content/10.110…
Today, we share a step in this direction:
🔹 A workflow supporting the analysis of 1,130 single-cell samples per day depth and quantitative accuracy.
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biorxiv.org/content/10.110…
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Parallel Squared Technology Institute (PTI) retweeted
An excellent discussion on building bio models by @alexrives on the @CogRev_Podcast
The emphasis on data -- not just volume but also type and quality -- resonates with our view and the approach @ParallelSqTech is taking.
youtu.be/l6DPAwgUr-8?si…
The emphasis on data -- not just volume but also type and quality -- resonates with our view and the approach @ParallelSqTech is taking.
youtu.be/l6DPAwgUr-8?si…
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Parallel Squared Technology Institute (PTI) retweeted
Don't correlate is a weak statement, especially in the absence of independent measurements & reliability accounting.
What's compelling for me are direct measurements of what regulates protein abundance.
doi.org/10.1101/2025.0…
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What's compelling for me are direct measurements of what regulates protein abundance.
doi.org/10.1101/2025.0…
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For everyone chiming in with "RNA and protein don't correlate"… here's some thoughts I wrote up on this point a while back:
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Parallel Squared Technology Institute (PTI) retweeted
Proteomics has long faced a choice: Go deep, or go fast.
Deep, high-confidence coverage of protein sequences has traditionally required extensive chromatographic fractionation, large sample inputs and data acquisition times too long to be practical for analyzing human cohort data-independent acquisition (DIA) workflows handle many samples quickly, but detect less of each protein and with lower confidence.
Our new preprint introduces multiDIA: It gets more from each sample without fractionation.
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doi.org/10.64898/2026.…
Deep, high-confidence coverage of protein sequences has traditionally required extensive chromatographic fractionation, large sample inputs and data acquisition times too long to be practical for analyzing human cohort data-independent acquisition (DIA) workflows handle many samples quickly, but detect less of each protein and with lower confidence.
Our new preprint introduces multiDIA: It gets more from each sample without fractionation.
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doi.org/10.64898/2026.…
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Parallel Squared Technology Institute (PTI) retweeted
Using single-cell proteomics, we discovered protein (and functional) symmetry breaking in early mammalian embryos influencing cell fates.
These proteomic asymmetries are detectable at the zygote stage, intensify by the 2-cell and 4-cell stage, and correlate with the sperm entry lization as a symmetry-breaking event. The clear protein differences allowed us to define two types of blastomeres, termed alpha and beta.
⬛ These differences predict the developmental potential of the blastomeres. Similar clustering and protein enrichment patterns found in human 2-cell embryos suggest this early asymmetry might be conserved.
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These proteomic asymmetries are detectable at the zygote stage, intensify by the 2-cell and 4-cell stage, and correlate with the sperm entry lization as a symmetry-breaking event. The clear protein differences allowed us to define two types of blastomeres, termed alpha and beta.
⬛ These differences predict the developmental potential of the blastomeres. Similar clustering and protein enrichment patterns found in human 2-cell embryos suggest this early asymmetry might be conserved.
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Single-cell proteomics is beginning to reveal functional coordination and principles.
This motivates us to keep advancing the depth, accuracy, and scalability to further empower this analysis and help identify biological principles and targetable disease mechanisms.
This motivates us to keep advancing the depth, accuracy, and scalability to further empower this analysis and help identify biological principles and targetable disease mechanisms.
Beyond heterogeneity, single-cell analysis can reveal functional coherence.
Coherence, regularity and principles advance science.
Full recording: youtube.com/watch?v=z0dIhG…
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Parallel Squared Technology Institute (PTI) retweeted
Beyond heterogeneity, single-cell analysis can reveal functional coherence.
Coherence, regularity and principles advance science.
Full recording: youtube.com/watch?v=z0dIhG…
Coherence, regularity and principles advance science.
Full recording: youtube.com/watch?v=z0dIhG…
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Parallel Squared Technology Institute (PTI) retweeted
Kicking off our first quarterly update from Parallel Squared Technology Institute (@ParallelSqTech): A boost in the throughput and quantitative accuracy of single-cell proteomics, and a warm welcome to our new Chief of Staff.
Mass spectrometry based single-cell proteomics has mpossible" to well demonstrated set of methods measuring thousands of proteins per cell, as traced in a recent Nature Technology Feature.
A next big challenge: getting to higher throughput without losing depth or quantitative accuracy.
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Mass spectrometry based single-cell proteomics has mpossible" to well demonstrated set of methods measuring thousands of proteins per cell, as traced in a recent Nature Technology Feature.
A next big challenge: getting to higher throughput without losing depth or quantitative accuracy.
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Great to see multiplexed single-cell proteomics uncover new biology. We are looking forward to further increasing the plex and thus the power of the technology for biological discoveries!
Congratulations to Luke Khoury and team !
Congratulations to Luke Khoury and team !
Millions of PBMCs have been analyzed by scRNA-seq. Have we seen it all ?
We profiled thousands of single PBMCs — proteomes and transcriptomes side by side — and uncovered functional coordination undetectable in the deepest scRNA-seq we could perform.
biorxiv.org/content/10.648…
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Parallel Squared Technology Institute (PTI) retweeted
Millions of PBMCs have been analyzed by scRNA-seq. Have we seen it all ?
We profiled thousands of single PBMCs — proteomes and transcriptomes side by side — and uncovered functional coordination undetectable in the deepest scRNA-seq we could perform.
biorxiv.org/content/10.648…
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We profiled thousands of single PBMCs — proteomes and transcriptomes side by side — and uncovered functional coordination undetectable in the deepest scRNA-seq we could perform.
biorxiv.org/content/10.648…
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Parallel Squared Technology Institute (PTI) retweeted
Among the numerous metrics evaluating academics, one stands out for me:
Our intellectual heritage reflected in our students and colleagues, and their intellectual growth.
That amplifies the initial sparks and bears more fruit than any one of us could have produced.
Our intellectual heritage reflected in our students and colleagues, and their intellectual growth.
That amplifies the initial sparks and bears more fruit than any one of us could have produced.
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Parallel Squared Technology Institute (PTI) retweeted
Excited to see what @slavov_n and @ParallelSqTech have been working on in @Nature's new article on single-cell proteomics.
Nature just published a tech feature on single-cell proteomics.
It highlights our journey from skepticism to robust technology and biological discoveries.
I love that the feature starts with a biological discovery enabled by the technology: cell.com/cell/fulltext/…
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Parallel Squared Technology Institute (PTI) retweeted
The piece revisits the early days, our lab's initial work alongside other groups pioneering complementary approaches, together showing that mass-spec-based single-cell proteomics was feasible & promising. The proof-of-concept set the field in motion.
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nature.com/articles/d4158…
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nature.com/articles/d4158…
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Parallel Squared Technology Institute (PTI) retweeted
Nature just published a tech feature on single-cell proteomics.
It highlights our journey from skepticism to robust technology and biological discoveries.
I love that the feature starts with a biological discovery enabled by the technology: cell.com/cell/fulltext/…
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It highlights our journey from skepticism to robust technology and biological discoveries.
I love that the feature starts with a biological discovery enabled by the technology: cell.com/cell/fulltext/…
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