New pre-print(s) from the Sternberg Lab in collaboration with @LeifuChangLab! We uncover an unprecedented molecular mechanism of CRISPR-Cas12f-like proteins, which drive RNA-guided transcription independently of canonical promoter motifs.

Full story here: tinyurl.com/mr3fd493

Our new preprint is live! doi.org/10.1101/2025.0…
In collaboration with @SternbergLab, we determined the cryo-EM structure of a dCas12f-σE-RNAP complex from F. taeanensis, revealing an RNA-guided transcription mechanism that bypasses canonical promoter motifs.

@SternbergLab Collectively, this work provides high-resolution insights into an unexpected mechanism of RNA-guided transcription, expanding our understanding of bacterial gene regulation and opening new avenues for programmable transcriptional control.

@SternbergLab Fun collaboration with @SternbergLab, and huge thanks and congrats to Renjian, Florian, Dan, Tanner, Adriana, and the whole team!